Increased binding of actinomycin D to halodeoxyuridine-substituted DNAs.
نویسندگان
چکیده
منابع مشابه
Increased binding of actinomycin D to halodeoxyuridine-substituted DNAs.
We have used equilibrium dialysis and other techniques to examine the actinomycin D binding sites on unsubstituted and halodeoxyuridine-substituted DNAs. Some of the actinomycin D binding sites present on halodeoxyuridine-substituted DNAs have an enhanced affinity for actinomycin D compared to the standard binding sites present on unsubstituted DNA. Kinetic studies indicate that actinomycin D d...
متن کاملThe nature of actinomycin D binding to d(AACCAXYG) sequence motifs.
Earlier studies by others had indicated that actinomycin D (ACTD) binds well to d(AACCATAG) and the end sequence TAG-3' is essential for its strong binding. In an effort to verify these assertions and to uncover other possible strong ACTD binding sequences as well as to elucidate the nature of their binding, systematic studies have been carried out with oligomers of d(AACCAXYG) sequence motifs,...
متن کاملIncreased susceptibility to staphylococcal enterotoxin B intoxication in mice primed with actinomycin D.
Mice (BALB/cJ, C3H/HeN, and C3H/HeJ) primed with actinomycin D became highly susceptible to lethal intoxication with staphylococcal enterotoxin B (SEB). The mice underwent toxicosis and toxic shock and died. Actinomycin D-primed C3H/HeN and C3H/HeJ mice showed equal sensitivity to SEB, suggesting that bacterial lipopolysaccharide derived from gram-negative bacteria in the gut may not be an impo...
متن کاملAssociation of actinomycin D and deoxyribodinucleotides as a model for binding of the drug to DNA.
The association of actinomycin D and eleven different deoxyribodinucleotides were studied as model complexes for the interaction of actinomycin D and DNA. All of the deoxyribodinucleotides containing guanine will complex with actinomycin D, and the guanine base has a preferred orientation with respect to the chromophore of actinomycin D. The spectrophotometric binding curve for pdG-dC is sigmoi...
متن کاملThe Binding of Actinomycin D-3h to Heterochromatin as Studied by Quantitative High Resolution Radioautography
A few studies have been carried out on the intracellular distribution of actinomycin DH all of them at the level of light optical radioautography since the specific activity of the labeled antibiotic did not permit its ultrastructural localization. These studies have shown that actinomycin D-3H is located in the nucleus (1, 4, 8) and is associated with the deoxyribonucleoprotein fraction (10) a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1979
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30037-6